author(s)
Anand Kumar, RK Gupta and VP Sabhlok

abstract
The present experiment was carried out in laboratory of department of zoology and aquaculture in CCS HAU, Hisar. The fresh water fish Cirrhinus mrigala were exposed to two sub lethal concentration of zinc and lead of three-dose levels i.e 0.01, 0.02, 0.04 ppm for 21 days with ascorbic acid feeding at the rate of 400 mg/kg of diet to find out the ameliorating effects of the ascorbic acid. The ascorbic acid content were analyzed and found decrease with an increase of the dose level of lead. It was observed 35.05% decreased at 0.01pm 43.38 % at 0.02ppm and 66.85 % at 0.04 ppm as compared to the control while in case of zinc treatment 66.07 % at 0.01ppm, 72.20 % at 0.02ppm and 84.04 % at 0.04 ppm as compared to the control and also showed 8% liver protein decreased at 0.04 ppm of lead, while serum protein decreased 11 % less as compared to the normal feeding experiment. In zinc treatment it was noted that 5 % liver protein decreased less but 10 % serum protein decreased less at 0.04 ppm dose level as compared to the normal feeding. The cholesterol level also increased less. In zinc treatment it was increased 17% less at 0.04 ppm but in lead treatment cholesterol level increased 22% less as compared to the normal feeding.