author(s)
Sindhu R, Swarnakumari N, Thiribhuvanamala G and Shanthi A

abstract
Nematode egg parasitic fungus, Pochonia chlamydosporia (TNAU Pc001) is widely utilized as a biocontrol agent in nematode management. P. chlamydosporia is endophytic in nature but also lives saprophytically in the soil when the host nematode is absent. Therefore the present study was undertaken to investigate P. chlamydosporia colonization in different soil type and various depths. An experiment was conducted to identify colonization pattern in different types of soil. Pot mixture (Red soil, Sand, FYM at 2:1:1) served as control. The results revealed that clay (Cotton) soil showed highest colonization (6.53×10⁷ cfu/g). Another set of experiment was conducted to assess the depth of colonization in different soils in test tubes and samples were plated from different depth (2 cm, 4 cm and 6 cm). The results indicated that the CFU was found higher in clay (Rice) soil (5.78×10⁶ cfu/g) at 6 cm depth compared to control. Colonization was found up 6 cm in all type of soil.