Leptin hormone diversity in different species-explained with polymorphism study in Pandharpuri buffalo
VR Patodkar, PV Mehere and SN Jadhav
Buffaloes constitute 35% percent of the total bovine population in India and contribute in the 53 % of total milk production. Current technologies enable scientists to improve the accuracy and efficacy of traditional selection by applying the genetic markers. Leptin gene is one of the markers which have been established for milk traits in cattle. But our recent research in buffalo shows that there may be other markers or the sites which may define the milk traits in animals.
This study was aimed to reveal PCR-RFLP pattern of the leptin (exon 2) locus in the Pandharpuri breed of buffaloes. Fragments of leptin 331 bp and OLR1-288bp were amplified by PCR, and subsequently RFLP study was carried out to identify genotypes of the animals with HphI restriction enzyme and HaeIII restriction enzyme to know the relationship of polymorphism in Exon 2 of leptin gene with milk traits but no such relationship has been found. The monomorphic pattern of leptin gene was observed in the present study for exon 2 in Pandharpuri Buffaloes.
VR Patodkar, PV Mehere, SN Jadhav. Leptin hormone diversity in different species-explained with polymorphism study in Pandharpuri buffalo. J Entomol Zool Stud 2019;7(3):414-416.