author(s)
PR Rathod, RS Ingole, VC Ingale, SV Kuralkar, SJ Manwar, SW Hajare, MV Ingawale and PN Rathod

abstract
The present study was to identify the E. coli collected from Akola district. Twenty one water samples were collected for morphological, bacteriological and biochemical investigations for isolation and identification of E. coli. The samples were cultured on nutrient broth and after culturing the same samples transferred to specific media EMB & MC agar. Biochemical properties of the isolates were considered and response in TSI test incline was likewise watched. The samples which give particular morphological and staining characteristic were chosen for the further biochemical examination. From the present study it was concluded that the general prevalence of E. coli was recorded as 66.66% from the poultry farms. The outcomes recommended that water samples collected from the various farms were shown to be a significant wellspring of E. coli infections in the field conditions. The presence of E. coli at the recorded rate in water samples poses significant alarming for the public health issue if not maintain proper hygienic steps taken place.