author(s)
M Bhubaneshwari Devi, Vivekananda Yaikhom and Dhananjoy Singh Chingangbam

abstract
SCoT (Start Codon Targeted) is a novel method for generating plant DNA marker. It had been using to determine the genetic polymorphism or to delineate the species of plants. In the present study the primer has been utilized to evaluate the genetic variability of five Chironomus species for the first time from Manipur. The larvae from different breeding ground of aquatic habitats were collected and reared till adults emerged. The identification of the species was done from larvae, exuviate and adults. DNA were extracted from either larvae or exuviate or adults. The COX 1 primer used for the sequencing produced around 700 base pair. The primer of the SCoT I was used to run PCR and product were run gel in 2% agarose gel for one and half hour in duplicate. The countable bands were recorded for the analysis. The bands were recorded and analysed using NTSYS pc to construct the phylogenetic tree. This plant primer has been utilized in studying the polymorphism in insects Chironomus and SCoT I produces promising polymorphic bands as well as non-polymorphic bands. The future works should have focused on the increasing number of the primers (15 to 20) and the specimens for the study should be more so as to produce a promising results to evaluate delineate the species of the Chironomus more precisely and less laboriously as DNA sequencing and the phylogenetic studies. On the basis present results, it can be safely said the SCoT could be used for the evaluating the genetic variability of Chironomus in future.