author(s)
Ramkaran, Anita Ganguly, Sandeep Gera, Preeti, Ankit Magotra and Sandeep Kumar

abstract
DGAT 1 enzyme catalyses the final step of milk triglyceride biosynthesis. A lysine to alanine substitution (K232A) at nucleotide position 10433-10434 at DGAT 1 is significantly associated with milk production traits. This polymorphism may potentially be used as a marker in selection programmes to enhance the production potential and to accelerate the rate of genetic gain. In the present investigation, DGAT1 K232A polymorphism was analysed in crossbred Hardhenu (HF X SW X Haryana) cattle (n=50) by restriction digestion of a 413 bp PCR fragment by Eae I. An undigested fragment of 413 bp indicated the K allele whereas two co-migrated fragments of 210 and 203 bp length indicated the A allele. In Hardhenu cattle population genotype frequencies were 0.30 (KK) and 0.70 (AK). The frequency of K and A allele were 0.65 & 0.35. Over all 15 KK and 35 AK animals were recognised in Hardhenu cattle whereas no animal of AA genotype were observed. The overall DGAT1^K allele frequency in crossbred cattle was 0.65.