author(s)
Archana Anokhe, Bikash Mandal, Raju Kumar, Kirti Sharma, Ranjit Ranjan and Sabtharishi Subramanian

abstract
Whitefly, Bemisia tabaci (genn.) is polyphagus pest causes severe damage to crops, by sucking the sap from plants and as vector of several viral diseases. B. tabaci is considered as a species complex of 35 genetic groups recorded so far. Key digestive enzyme lipase was investigations under this study in host specific male and female populations of B. tabaci collected from different agro-ecological zone. From the study it was found that Sri Ganganagar population has shown significantly higher lipase activity than Delhi and Coimbatore. Turkey grouping of crop mean comparison revealed that lipase activity was significantly higher in female population reared on host tomato (4.86A µmol/ml/mg) than cotton (3.52B µmol/ml/mg) and brinjal reared population (2.07C µmol/ml/mg). Tomato reared population exposed to Tomato Leaf Curl New Delhi virus and discriminating dose (781mg/ml) of insecticide Imidachlorprid (99%) to see the effect of virus and insecticide in dynamics of lipase activity. From result it was found that lipase activity is high in virus acquired population as compare to survival of insecticide exposed and control populations, which indicate that lipase play important role in virus vector relationship. Biochemical characterisation of metabolic enzymes would improve our understanding on the metabolic capabilities of host specific differential lipase activities vis-a-vis host plant adaptations and management of B. tabaci species complex in crops.