author(s)
KK Khose, SJ Manwar, ED Sedmake, RS Ingole and PR Rathod

abstract
The present study was carried out to evaluate the effect of supplementation of herbal choline in diets on haematological indices and development of vital organ in broilers. Total three hundred Cobb-400 straight-run day-old broiler chicks were randomly distributed into five treatment groups with three replicates containing 20 chicks in each and reared up to 6 weeks. The group T1 (control) offered basal diet, while group T2 basal diet with choline chloride-60% @ 1 kg/ton of feed, groups T3, T4 and T5 offered basal diets with herbal choline @ 0.250, 0.350 and 0.500 kg/ton of feed, respectively. The results revealed that the values of haematological parameters at 21^st day as well as internal organ weights at 42^nd day in all treatment groups were statistically non-significant. The treatment group T5 showed significantly (P<0.01) higher hemoglobin values when compared with groups T1, T3 and T4 groups at 42^nd day. The mean values of mean corpuscular haemoglobin concentration in T2 and T5 were significantly (P<0.01) increased as compared to control group. The total leucocyte and lymphocyte count was found to be significantly (P<0.01) increased in treatment groups T2, T4 and T5 as compared to T1 and T3. It is thus concluded that the broiler diet supplemented with synthetic choline or herbal choline did not alter the haematological parameters at the 21^st day and internal organ weight at 42^nd day. However, at 42^nd day the haematological values of haemoglobin mean corpuscular haemoglobin concentration, total leucocyte count and lymphocytes were significantly increased in treatment groups supplemented with synthetic choline (1 kg/ton) or herbal choline (0.500 kg/ton) compared to control group. Thus, the supplementation of synthetic choline @ 1 kg/ton or herbal choline @ 0.500 kg/ton in broiler diets showed beneficial effect on haemopoiesis.