author(s)
Amita Tiwari, PC Shukla, Devendra Gupta, Varsha Sharma and RPS Baghel

abstract
Among various goat diseases, mycoplasmal infections are one of the important infections. Clinical mycoplasmoses often lacks pathognomonic characteristics, so definitive diagnosis of the disease is quite cumbersome. Present study was conducted on mastitic milk samples of 215 goats to detect Mycoplasma agalactiae using Polymerase chain reaction (PCR). Results revealed that, out of 215 milk samples, a specific 176 bp bands obtained from the DNA amplification of Mycoplasma agalactiae using primers ma-mp 1F and ma-mp 1R in 31 samples. This confirms that 14.42% of mastitic goats were suffering from Mycoplasma agalactiae infection.