author(s)
Vijay K Kadam, Abhijit K Barate, Tejas C Shende and Hemant S Birade

abstract
Follicle-stimulating hormone (FSH) plays a pivotal role in the reproduction of mammals. In this study, partial FSHB-3 of HFxS 5041 crossbred bull has been characterized and analysed for the presence of single nucleotide polymorphisms (SNPs). Analysis revealed that HFxS 5041 FSHB-3 has 99.1% homology at the nucleotide level and 98.6% homology at the amino acid level with cattle FSHB-3 sequences. Two SNPs were detected in the FSHB-3 sequence of HFxS 5041 (4453A>G and 4489A>C) compared to cattle M83753 sequence. Amongst the two, the novel SNP 4453A>G led to amino acid change Ser103Gly. Future study with larger number of bulls having Ser103Gly mutation is necessary to confirm the role of this substitution on fertility of crossbred bulls.