author(s)
VP Saini, Alok D Kamble, ML Ojha and Surnar Sharad Raosaheb

abstract
Clove oil was applied as an aid in seed transportation in plastic bags. The safe concentrations of clove oil (0.02 ppm) calculated using second order polynomial regression was used for this purpose .This concentration was applied for 24, 48 and 72 hrs of transportation with three loading densities of 1000, 1500 and 2000 fish per bag of 3,000 ml freshwater. In 24 hrs transport at high packing density (2000 seed /bag) without clove oil, mortality rate was only 2%. However, there was no mortality during 24 hrs transport in clove oil treatments. In clove oil treated fish there was no mortality upto 48 hrs transportation and at 72 hrs transport only 3.70% mortality was noticed. Whereas in control 3.4 to 58.3% mortality was observed. During transportation experiments a significant reduction in OCR, COR and ammonia excretion rate was also noticed in clove oil treated seed bags.