author(s)
Sana KM Patel, ST Indulkar, Abdul Lateef AH Shaikh, MS Sawant, AS Pawase and RK Pai

abstract
In the present study, effects of various concentrations of ethephon exposures on days 7, 14, 21 and 28 for many serum biochemical parameters in the freshwater teleost fish, Pethia conchonius were investigated. The 96h LC₅₀ value of ethephon was estimated by using log-dose probit regression line method. On the basis of LC₅₀ value, the sub-lethal concentrations were determined as 6.40 and 12.81 mgl^-1 which is 1/10^th and 1/15^th of LC₅₀ respectively. Well acclimated fishes from both control and treated group were sacrificed after 7, 14, 21 and 28 days and blood samples were collected. Various biochemical parameters such as serum total protein, serum albumin, serum globulin, and serum glucose has been studied as diagnostic tools. In general significant effects (p<0.05) from different concentrations and time of exposure were observed in exposed fishes. It was found that significant alterations in all the biochemical parameters were dose dependant as well as duration dependent. Results indicated that serum albumin, serum globulin and serum total protein decreased significantly where as serum glucose increased with increase in ethephon concentration and time of exposure when compared with control groups.