author(s)
OS Kashikar, AR Deshpande, PR Suryawanshi, SS Gaikwad and SS Kashikar

abstract
Rotaviral infection is a viral disease, caused by Rotavirus belonging from Reoviridae family. Young ones of animals and human are most susceptible to this disease. However, adult animals and human with infection may also show similar signs at some extent. The disease is infectious-communicable among buffalo and human, there is a wide range of morbidity and mortality. In the present study, 49 faecal samples from buffalo and 55 stool samples from human, showing the signs and symptoms related to Rotavirus infection were collected. It was observed that out of 49 faecal samples from buffalos, 10 samples and out of 55 human stool samples, 21 samples were found positive for RNA-PAGE. Representative PAGE positive fecal and stool samples, 6 each were selected for RT-PCR and out of 6 fecal samples, 3 samples were found positive and out of six stool samples, 3 samples were confirmed positive for Rotavirus infection. One sample from buffalo and one from human were sent for sequencing. The phylogenetic analysis was carried out and it was confirmed that the positive samples were showing relatedness with the Rotavirus sequences taken from GenBank. As per the sequences brought, it was confirmed that the virus was originating from the eastern part of India (West Bengal). Therefore it is indicative of circulation of virus from state to state in Indiacan be possible. The buffalo sample was showing relatedness with human rotaviral sequence, which is indicative of sharing of Rotavirus between human and animal.