An investigation was carried out during 2015-16 and 2016-17 using five biocontrol agents along with chlorpyriphos 20 EC @ 2ml/lit as insecticidal check, against O. obesus in preserved setts of sugarcane in Jorhat, Assam. All the treatments were significantly superior over untreated control which recorded the highest level of infestation and poor germination percentage. Among the biocontrol agents, Metarhizium anisopliae @ 1012 spore/lit treated setts gave promising results and recorded lowest mean number (21.25 and 19.06%) and portion of setts (22.62 and 23.59%) infestation with germination percentage of 67.86 and 67.81 during 2015-16 and 2016-17 respectively. Insecticidal check chlorpyriphos treated preserved setts maintained its superiority by registering least mean number (15.16 and 14.84%) and portion of setts (14.81 and 16.10%) infestation coupled with a marked increase in per cent germination (78.02 and 76.82%) during both the years.