author(s)
Manju Lata Mishra, Sangita Sood, Vikram Patial, Anil Sood, Bikram Singh and UN Shukla

abstract
Histo-pathological assessment of tatwakhar i.e. processed flour of Aesculus indica of different proportion diet was given to the fifty weanling rats weighing 25±5g, which were divided into control, control with diabetic, and three experimental groups, the trial diet consist of 25, 50 and 75 percent tatwakhar or processed flour. It was given in powdered form weighing daily 10 g for 40 days. Thereafter the rats were sacrificed and fixed immediately in buffered formalin 10(%), carried to the laboratory, where it was processed to paraffin wax, cut at 3-5 microns, stained by using Hematoxylin and Eosin technique and analyzed their organs viz. heart, liver, kidney, and pancreas under computerized microscope. There was mild congestion and increased interstitial space in heart, increased cytoplasmic granularity in liver, moderate congestion and mild tubular swelling in kidney and mild congestion in pancreas was found in rat having 25 percent tatwakhar flour with diabetic group. After giving 50 percent experimental diet, the result showed no congestion, mild increase in interstitial space in heart, mild congestion, increased cytoplasmic granularity, mild tubular swelling and congestion in kidney and reduction in islet of Langerhans in pancreas. When 75 percent tatwakhar flour with diabetes was studied, muscle fiber was more eosinophillic, cell inflammation and necrosis in heart, mild cellular swelling and necrosis in liver, mild tubular swelling and congestion in kidney and full developed islet of Langerhans maximum secretion of insulin hormone in pancreas. It might be due to the presence of aescin in the flour. These findings could suggest that the maximum use of tatwakhar flour is good effect on pancreas as well as on other organs.