author(s)
Pankaj Jain, Apra Shahi, AP Singh, Madhu Swamy and Rakhi Vaish

abstract
A modified method for decellularization of buffalo diaphragm was developed to prepare an acellular biological scaffold which can be utilized for repair of varied muscular defect in both large and small animals of different species. The method allowed an efficacy of 94.61 % for decellularization when 1% SDS was used as detergent. Histology, nuclear staining and gel electrophoresis confirmed the acellular nature of scaffold. Keeping quality of scaffold was excellent even after storage of 12 months before clinical use. Such material will reduce the dependency over tissue donor and percentage of graft rejection.