author(s)
Richa Arora, Arpit Tyagi, S Shekhar, VS Rajora and Niddhi Arora

abstract
The present study was conducted in the Department of Veterinary Medicine, Teaching Veterinary Clinical Complex and Instructional Dairy Farm, C.V.A.Sc., GBPUA&T, Pantnagar, U.S. Nagar (Uttarakhand, India). A total of 30 buffaloes were included in the study out of which 24 were suffering from simple indigestion whereas 6 healthy buffaloes were chosen to serve as healthy control. In the buffaloes suffering from indigestion, rumen motility significantly reduced, pH of rumen liquor was slightly acidic and rumen liquor examination revealed reduced microbial count and activity, which was indicated by reduced total protozoal count, protozoal motility, bacterial count and amount of Total Volatile Fatty Acids and increase in time values for Methylene Blue Reduction Test (MBRT), Sedimentation Activity Test (SAT) and Nitrate reduction test. Hemogram with leukocytosis, neutrophilia and lymphocytopenia was suggestive of stress like condition. Alterations in biochemical parameters included significant (P≤0.05) increase in serum Alaninie Amino Transferase (ALT), Aspartate Amino Transferase (AST), Lactate Dehydrogenase (LDH) and Blood Urea Nitrogen (BUN) concentrations and reduced serum glucose levels.