author(s)
Sadaf Sakeena, Rafiqul Islam, Qumaila Sakeena, MD Moin Ansari, Arjuma Khatum, Asloob Malik, FA Lone and BA Moulvi

abstract
The present study was conducted at Faculty of Veterinary Sciences and Animal Husbandry, Shuhama to determines the alterations in leukocyte and biochemical constituents including oxidant-antioxidant indices in the blood serum of cyclic and acyclic ewes with or without uterine infection. Both infectious and normal ewes were further sub divided into Follicular infectious (FI), Luteal infectious (LI), Follicular normal (FN), Luteal normal (LN) and Acyclic normal (AC). The mean pH of the uterine secretions obtained from ewes suffering from uterine infection was significantly higher (P<0.001) than normal ewes. Neutrophils and TLC in the infectious group was significantly higher than in the normal group of ewes. The NO concentration in follicular fluid was significantly higher (P<0.001) in FI (56.91±11.94) and LI (51.25±8.75) group than FN (11.64±0.96), LN (14.79±2.40) and AC (18.67±1.13) group of ewes. Follicular ascorbate was significantly lower (P<0.05) in FI (30.85±6.28) than FN (73.20±12.16) and AC (63.05±15.09) ewes. Follicular cholesterol concentration was significantly higher (P<0.05) in LI (133.03±1.25) than FN (129.16±0.44), LN (129.96±0.24) and AC (129.42±0.51) ewes. Total protein concentration (g/dl) was significantly higher (P<0.05) in LN than both luteal group of ewes (LN and LI). The mean concentration of serum MDA is shown in Table 8 and Fig 9. The circulatory MDA level (nmol/L) was significantly higher (P<0.05) in FI (1049.32±25.64) and LI group (1030.25±26.69) than AC (952.95±15.55) group of ewes. The Serum Mn level was significantly higher (P<0.05) in LI (7.31±0.49) and LN (6.26±1.13) than FI (3.01±0.98) group of ewes. In general, the zinc concentration was observed to be higher in cyclic normal ewes as compared to cyclic infectious ewes.