author(s)
Akhilesh Pandey and MS Thakur

abstract
The present study was carried out in the Department of Animal Genetics and Breeding of College of Veterinary Science and A H Jabalpur. According to the research finding populations of 50 Malvi and 50 Nimari cattle were found to be monomorphic after PCR-RFLP profile at the αs1-casein gene (CSN1S1) locus. Its monomorphic properties revealed that only one type of uncut banding pattern (AA genotype); which was of 310 bp. No any animal observed with AB and BB genotypes in above two breeds of cattle. The genotypic frequency AA was 100 per cent in screened animals for present investigation in above mentioned both breeds of cattle. The allelic frequency for allele A in the screened animal of Malvi and Nimari cattle was 1.00 and for allele B was 0.00 in investigation. Chi-square values were found to be non- significant in both Malvi and Nimari population, indicating that the populations of these two cattle breeds under were in Hardy-Weinberg equilibrium (HWE) at this locus. Association of αs1-Casein (CSN1S1) gene polymorphic variants with milk production traits revealed that there is no significant difference in the milk production per lactation by the AA genotype of Malvi and Nimari but Nimari showed significantly higher Daily milk yield in AA genotype than Malvi.