author(s)
S Das, M Medhi, M Khaound, P Doley, M Islam and DP Borah

abstract
In the present study, electropherotyping and reverse transcriptase polymerase chain reaction (RT-PCR) were applied for diagnosis of group a rotaviral infection in diarrhoeic clinical samples of bovine (175) randomly collected from different parts of Assam, India. Electropherotyping analysis revealed that, RNA of 24 numbers of samples showed 4-2-3-2 pattern migration of segments on gel with 13.71% positivity. All the samples were also screened by RT-PCR. For RT-PCR two published primer sets were used which were amplified to produce 309 and 304 bp sized amplicon for VP6 and VP7 gene on 1.7% agarose gel electrophoresis and 39 (22.28%) numbers were found to positive for both VP6 and VP7 genes of bovine rotavirus. Both Electropherotyping and RT-PCR were suitable for detection of group a rotaviral infection though RT-PCR was found to be more sensitive than electropherotyping.