author(s)
Saurabh, Sushant Srivastava, Pushkar Sharma and Vijay Gautam

abstract
The present study was carried out to evaluate the influence of ascorbic acid (@ 2mM after incorporation in on quality of spermatozoa after storage of epididymis at temperature 4 ⁰C & -196 ⁰C. Cyto-morphological characteristics of epididymal semen from forty testes stored at room temperature, refrigerated and and -196 ⁰C were evaluated). Similarly, tris dillutor with ascorbic acid were infused inside the ligated epididymal part cauda and stored at 4 ⁰C (T₂) and -196 ⁰C (T₄) and further evaluated for cryoprotectant efficacy of ascorbic acid. A significant variation in initial motility, viability, and abnormal sperm count were observed among the groups. In present study the motility of spermatozoa was significantly (P<0.01) positively correlated with live count per cent and HOS reactive spermatozoa per cent, whereas, it is negatively correlated with sperm abnormality per cent. On histological examination of epididymis revealed that cryoinjury during storage at low temperature might be a cause of poor post-thaw recovery in T3 and T4 treatment. On the basis of present study it can be concluded that cryopreservation of whole epididymis may be utilized as one of the potential method for storage of epididymal spermatozoa and ascorbic acid as an additive have a promising results in cryopreservation of epididymal semen.