author(s)
May Naji Al-Khanaq

abstract
The present study was intended to detect the cutaneous Leishmaniasis from a total of suspected cases that were gathered from dermatology center of AL-Zahra'a and AL-Karamah Teaching Hospital in Al-Kut city from October 2015 to the end of February 2016. Totally 70 patients aged 1<45 year were included 30 male, 40 female. The collected wounds specimen were looked at with microscope examination of smears stained with Giemsa staine and genospecific Nested PCR, Of 70 patients microscopy uncovered amastigotes in 21(30%), while Nested PCR identified Leishmania in 55(79%) positive samples. Nested PCR revealed a total of 55 very much archived cases in the present study 49(70%) were brought on by Leishmania major and 6(9%) by Leishmania tropica. There was a critical relationship between the conveyances of cutaneous Leishmaniasis among patients as indicated by sex, age and habitation. Settled PCR offers fast ID of Leishmania to the perplexing level.