author(s)
Adil Abbas Alsultani, Luma Waleed Khalil and Sabah Mahdi Hadi

abstract
This study was designed to assess ameliorative role of casein Hydrolysate specially bioactive peptide on hyperlipidemia that induced by fructose in adult male rats. Thirty two male rats were randomly and equally divided into four groups for 42 days. Experimental groups were as following: group one (G1) control group, group two (G2) treated with casein Hydrolysate (1g/kg/day) orally, group three (G3) treated with fructose (40%) in drinking water and group four (G4) given casein Hydrolysate and fructose. Blood sample collection from orbital sinus technique by using capillary tube in case anesthetized rats after 42 days of the experimental period for measuring the following parameters: triglyceride, total cholesterol, high density lipoprotein cholesterol, insulin-like growth factor-1, and DNA fragmentation. Results exhibited that there is a significant decrease (p > 0.05) in triglyceride and total cholesterol in casein Hydrolysate group and casein Hydrolysate with fructose group as compared with other groups. At the same time, high density lipoprotein cholesterol increase significantly (p > 0.05) in casein Hydrolysate group compared with other groups. DNA fragmentation of white blood cells of fructose group showed induction of chromosomal damage compared to casein Hydrolysate with fructose that ameliorates this damaging effects. In conclusion, the results of this study documented that casein Hydrolysate is a good hypolipidemic agent that will ameliorates the deleterious effects of fructose in adult male rats.