author(s)
Maria Kanan and Max Tulung

abstract
Flies are members of the Diptera order that many act as vectors of disease, in humans and animals. Fighting of the fly population starts from knowing the position of the species. Research has been conducted to know the position of flies species originating from several habitats in Luwuk City, Central Sulawesi. Three locations as source of fly sample are traditional market (PSR), settlement (RS1) and hospital (RS2). The thorax and back legs are used for DNA extraction and purification. Amplification of CO1 gene was done by PCR method. Visualiasi amplicon of CO1 gene is done by electrophoresis method. Nucleotide sequencing is done by sequencing method. The sequence of CO1 gene obtained in the BLAST analysis on the NCBI website and followed by phylogenetic reconstruction using the Geneous 10.1.1 Program and the MEGA 7.0 Program. The results showed that: PSR fly gene sequences have the highest similarity percentage with Chrysomya megacephala SCAU-DE-LD-C26 [KY020769] (99%). While the gene sequences of RS1 and RS2 CO1 each show the highest similarity with Chrysomya megacephala CSU160701CB14 [KY001899.1] (99%) and Hermetia illucens [HQ541186.1]. However, the reconstruction of the phylogenetic tree formed by RS1 and RS2 flies has an evolutionary relationship or similarity to Chrysomya megacephala Voucher CM1, whereas PSR has an evolutionary relationship or similarity to Chrysomya megacephala Haplotype SCAU-DE-LD-C26.